With an expansion of the FLUOVIEW imaging platform, the Olympus FV3000 confocal laser-scanning microscope offers two new upright models, designed for in vitro and in vivo experiments. The microscope’s resonant scanner captures video-rate confocal images without compromising the field of view at up to 438 frames per second. Olympus TruSpectral detection technology further improves imaging by combining high sensitivity and high accuracy for multichannel spectral imaging. The FV3000 microscope also makes it easy to view samples up close as well as in their larger context. With nosepieces that accommodate up to 7 objectives and an optimized macro-to-micro light path, users can capture images from 1.25X to 150X. These imaging features are now available in two new upright frames, one optimized for fixed tissue experiments and the other for electrophysiology.