SCIEX OS v3.0 is a software ecosystem designed to offer accessibility and integration as it powers mass spectrometers across the SCIEX portfolio. The system enables the delivery of quality data, improved workflow efficiencies, and auditing advancements. The software features stMRM, which utilizes the intelligent monitoring of marker compounds to trigger the acquisition of later eluting dependent transitions to identify dependent compounds. Removing analytical dependencies of chromatographic retention times, stMRM allows for improved cycle and dwell across compound transitions. The newly added Central Administration Console aims to bring project management, user profiles, permissions, and security into an easily navigable interface.