The Cellaca PLX image cytometry system from Revvity utilizes matrix analysis software and dedicated reagents and consumables to provide a benchtop solution for accurate measurements of small sample volumes. This capability allows for performance of rapid subpopulation analysis for downstream processing. The system is capable of simple yet sensitive cell counts, viability readouts, and multiplex analysis in seconds. It only requires between 15 and 50 µL per sample for analysis, and the time to downstream processing is shortened by multiplexing with four channels with viability readouts at one minute per sample. Optimized protocols streamline surface marker staining, vitality, and apoptosis analysis with step-by-step methodologies and customizable result presentations.