The 4D Multiomics timsTOF platform from Bruker has been expanded with the launch of the new timsTOF HT system. The system includes a novel 4th-generation TIMS (trapped ion mobility separation) XR cell and 14-bit digitizer for expanded dynamic range, enhanced peptide coverage, and more accurate quantitation, particularly in unbiased 4D plasma and tissue proteomics and epiproteomics. These advances are achieved without compromising the sensitivity of high-throughput proteomics, (eg, from 50 samples per day (SPD) up to 200 SPD), or a high scientific confidence of better than 1% peptide and protein FDRs (false discovery rates), avoiding inherent antigen cross-reactivity in targeted immune-recognition methods. The instrument can identify over 100,000 unique tryptic peptides in 60-min gradients with quantification of better than 5% CVs for many protein groups in low microgram quantities of a three-proteome mixture, using the dia-PASEF technique. The system also is capable of high-throughput, deep and unbiased plasma proteomics, and liquid biopsy biomarker research.